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To eliminate the possibility that other components in the WT SM influenced mutant growth, we tested the impact of exogenous GSH on growth using minimal media (solid and liquid)

Formula: Units = (Target Dose in mg / Concentration in mg per mL) x 100 Or equivalently: Units = (Target Dose in mcg / mcg per unit) Examples for a 10mg/mL solution: For 1mg dose: (1 / 10) x 100 = 10 units For 2mg dose: (2 / 10) x 100 = 20 units For 4mg dose: (4 / 10) x 100 = 40 units For 8mg dose: (8 / 10) x 100 = 80 units For 12mg dose: (12 / 10) x 100 = 120 units (requires two draws from a 1mL syringe) Step 6: calculate total doses per vial Divide the total vial potency by your weekly dose to find how many weeks one vial covers