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The reaction was carried out in 10l reaction volume containing 25 ng of RNA, 0.25 M of each primer, 0.125 l iScript Reverse Transcriptase enzyme (BIO-RAD, Hercules, CA, USA), and 5 l of 2x one-step SYBR Green Reaction mix (BIO-RAD, Hercules, CA, USA)

2D), implying that RSMC may function as a Sororin facilitator

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Both macroglia (astrocytes and Mller cells) and microglia, as well as infiltrating monocytes, are involved in the neuroinflammatory process and in the immune-mediated neurodegenerative process of RGCs that occurs in glaucoma, producing proinflammatory cytokines and complement components (Nickells et al., 2012