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We performed fecal microbiota transplantation to germ-free mice with feces from donors of two high-TMAO producers and two low-TMAO producers based on pre-supplement OCCT results (Figure S9a)

FLS were rinsed with PBS twice, and 2 mL of Rhodamine 123 solution (5 m) was added to each well and dyed at 37 C for 20 min in the dark

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Given the multifaceted nature of DN and its widespread implications, it is crucial for healthcare providers to recognize and address this condition through early diagnosis, effective pain management, and comprehensive care strategies aimed at improving patient outcomes and reducing the overall burden on public health systems ( 1.2 Pathophysiology and mechanisms of DN DN is a significant microvascular complication of diabetes mellitus, characterized by a range of nerve dysfunctions that can lead to debilitating symptoms such as pain, numbness, and other neurological impairments