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Importantly, these diet-induced changes in gene expression profiles were influenced by SNPs within the genes involved in choline metabolism, such as MTHFD1, CHDH , and PEMT ( Table 1 Human studies evaluating the effect of dietary choline

PMID: 29241711.

Besides, concerning about the environment protection, to ensure the yield of each synthetic process and to avoid an unaccepted intermediate entering into the next reaction during the multi-synthetic steps decreases the polluting steps and reduces the cost of the "three wastes" treatment

In casein, hydrolysis is enhanced in the substrate, which is evident by the presence of NH, OH, amide, COO, and P = O signals, characteristic of amino acids, in addition to the increase of the amide I and II bands