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Hashimotos disease is the most common form of hypothyroidism and was the first condition ever to be classified as an autoimmune disease

Wright et al., 2011)

Briefly, pinocytic cell loading reagent (Influx, Life Technologies) was prepared according to manufacturers instructions by heating reagent vials for 5 min to melt the PEG cap using an 80 C water bath, adding 4.7 ml of prewarmed culture medium (without serum) per vial, mixing vigorously at 37 C for 30 min to dissolve sucrose crystals, and then adding FBS (5% v/v) and sterile HEPES (10 mM)

This is one of the principles covered in our guide to common peptide storage mistakes using questionable BAC water is on that list for good reason