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Description

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For GST fusion proteins, cells were lysed by sonication in Buffer A (50 mM Tris-HCl, pH 8.0, 300 mM NaCl, 1 mM EDTA, 0.5% Triton X-100, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride/PMSF))