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monocytogenes that was rescued by either rha1 or rha2 expression in trans , demonstrating that both genes must be absent for the toxic effect to manifest (Fig 3D)

Topical antibiotics in the form of eye drops or ointments are often recommended for mild to moderate infections, while severe infections may necessitate intravenous antibiotics

Flow cytometry For CD3/CD4/CD8a antibody staining, 2 10 5 cells were preincubated with 2.5 l Human TruStain FcX Fc Receptor Blocking Solution (#422302, BioLegend, San Diego, CA, USA), followed by incubation with the designated antibody (Brilliant Violet 421 antihuman CD3 Antibody (#317344), PerCP antihuman CD4 (#300528), Brilliant Violet 421 antihuman CD8a (#301036), and the respective isotype controls (BioLegend, San Diego, CA, USA) for 30 min on ice while being protected from light

Copper ionophores demonstrate unique advantages in inducing cuproptosis, thereby positioning them as promising novel anticancer agents for the treatment of OSCC