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and Osbourn et al., Methods 36:61 -68 (2005) and Klimka et al., Br

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Fourth, verifying the correlation between combined markersincluding the abundance of specific genera (e.g., decreased Bifidobacterium), iron metabolism indicators (e.g., elevated serum hepcidin), and pro-ferroptotic metabolites (e.g., increased TMAO)and OA clinical phenotypes through large-sample clinical studies, and exploring their diagnostic or prognostic value