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Animals were housed in a specific pathogen-free (SPF) facility maintaining controlled environmental conditions: ambient temperature 22C 2C, relative humidity 50% 10%, and 12/12 h light/dark cycle with ad libitum access to standardized rodent chow and autoclaved water

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One theory behind premature hair graying suggests that a buildup of hydrogen peroxide in hair follicles due to decreased catalase activity might contribute to the bleaching of hair by reducing melanin production