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Construction of multiplemutant strains Construction of the potentially lethal strains completely blocked for the disulfidereducing pathways was accomplished as follows: a trxA , gshA strain was transduced with the lysate from the trxC strain described above, selecting for tetracycline resistance, on a plate containing a disk saturated with DTT

However, Borrelia spirochetes have an outer membrane architecture different from other Gram-negative bacteria and lacking endotoxin, i.e., LPS is not present (Ben-Menachem et al., 2003

Specifically, under section 1886(d)(12)(C)(i) of the Act, as amended, for FYs 2019 through 2024, a subsection (d) hospital qualifies as a low-volume hospital if it is more than 15 road miles from another subsection (d) hospital and has less than 3,800 total discharges during the fiscal year