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Dong X, Leppik IE, White J, Rarick J

Fresh leaf tissue (1.0 g) was homogenized in 10 mL of ice-cold 5% (w/v) meta-phosphoric acid containing 1 mM EDTA

Kitada M, Takeda A, Nagai T, Ito H, Kanasaki K, Koya D (2011) Dietary restriction ameliorates diabetic nephropathy through anti-inflammatory effects and regulation of the autophagy via restoration of sirt1 in diabetic Wistar fatty (fa/fa) rats: a model of type 2 diabetes