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The relative mRNA level of a specific gene including elastin ( ELN ), Nrf2 ( NFE2L2 ) and its downstream targeting genes is normalized to -actin, a housekeeping gene served as an internal control

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The heterogeneous sequences of minicircle DNA encodes guide RNAs that function in mRNA editing process (reviewed in Cavalcanti and De Souza, Crithidia fasciculata (a related, non-pathogenic trypanosome) (Tzfati et al., 1992) and more recently in T