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Fluorescence imaging For in vivo mRNA transfection, mice were intravenous injected with LNP@eGFP mRNA (1 mg/kg), and 24 h later all mice were euthanized, and their brains were separated and fixed in 4% paraformaldehyde for 24 h, dehydrated, frozen with OCT compound, and cut into 20 m frozen slices by a freezing microtome (Leica, Germany)

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