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488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays, Nudelman R, Kocks G, Mouton B et al (2023) The Nitrosamine saga: lessons learned from five years of scrutiny

KEGG pathway enrichment analysis showed that the DEGs were enriched in several immunologyrelated pathways (Figure 5D), such as Th17 cell differentiation [ko04659], Chemokine signaling pathway [ko04062], T cell receptor signaling pathway [ko04660], Th1 and Th2 cell differentiation [ko04658], Natural killer cell mediated cytotoxicity [ko04650], Leukocyte transendothelial migration [ko04670], B cell receptor signaling pathway [ko04662], Antigen processing and presentation [ko04612], NODlike receptor signaling pathway [ko04621], and Notch signaling pathway [ko04330]

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Cells are incubated a further 6 hours and then the Alamar Blue fluorescence intensity recorded using a Plasma and Metabolic Stability Each compound (1 M) is incubated with mouse plasma, for 4 hours at 37 C., with shaking at 100 rpm