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CB 1 receptor immunoreactivity was quantified from the optical density (OD) of DAB signal using color deconvolution paradigm in NIH Image J software ( Western Blot The frozen brain tissues were homogenized using syringe-based technique in 20 volumes of T-PER Tissue Protein Extraction Reagent and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific, United States)

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M.et al (2009)