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Description

TEV protease was purified using Ni 2+ -affinity chromatography and the eluted (His) 6 -tagged protein was used for biophysical studies

Bone-marrow cells were stained with the antibody mixture (lineage cocktail (Mac1 (CD11b), Gr1 (Ly-6G/C), Ter119 (Ly-76), CD3, CD4, CD8a (Ly-2), and B220 (CD45R)), c-Kit, Sca1, CD48 and CD150 antibodies) (BioLegend, 101226, 108424, 116223, 100222, 100414, 100714, 103224, 105812, 108120, 103404 and 115904) for 20 min at 4 C, washed and analyzed by flow cytometry