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Controlled human exposure studies have demonstrated that short-term exposure - up to 8 hours - causes lung function decrements such as reductions in forced expiratory volume in one second (FEV1), and the following respiratory symptoms: Cough Throat irritation Pain, burning, or discomfort in the chest when taking a deep breath Chest tightness, wheezing, or shortness of breath The effects are reversible, with improvement and recovery to baseline varying from a few hours to 48 hours after an elevated ozone exposure

Consistently, administration of MIF to mice following ischemic stroke disrupted tight junctions of the BBB and increased infarct volumes in vivo , which could be reversed by a MIF antagonist (Liu et al., 2018)