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Second, the GLDH enzyme uses oxidized cytochrome c as the only electron acceptor (Millar et al., 2003)

Saville et al., 2019

APAP hepatotoxicity occurs through formation of the noxious NAPQI metabolite, which is present in excessive quantities, as augmented by features of glutathione (GSH) depletion, oxidative stress and mitochondrial dysfunction leading to depletion in adenosine triphosphate (ATP) stores

The yield for all these combinations showed a broad range, indicative of the solubilization abilities of BugBuster alone to sonication in the presence and absence of IGEPAL CA-630 and NaCl