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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP

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Preparation of primary mouse thalamus explants and dissociated neurons All animal procedures were approved by the Animal Care and Use Committee (ACUC) of the National Heart, Lung and Blood Institute (NHLBI) animal protocol H-0331 in accordance with NIH research guidelines for the care and use of laboratory animals