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The chemiluminescent readout was obtained using SuperSignal West Femto maximum sensitive substrate (Thermo Scientific GmbH, Schwerte, Germany) with a c400 Azure imaging system (lowest sensitivity, 10 s exposure time)

However, studies reveal that p53 also plays a key role in energy metabolism, regulating glycolysis and oxidative phosphorylation to sustain energy demands and regulating intracellular ROS levels (Bensaad et al., 2006

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