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Previous studies found that in a cell-free media (that does not support growth), cysteine could partially substitute for GSH to promote Rickettsia infectivity 61 , aligning with our studies

Furthermore, a conjugate formation assay, utilizing Jurkat T cells, Raji B cells as APCs, and staphylococcal enterotoxin (SEE) as a TCR stimulant, followed by fluorescent cell staining and confocal microscopy confirmed that phospho-Thr 335 is required for PKC localization at the IS ( Figures 1I, J )

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The resulting strong inflammatory response promoted the formation of inducible TLS (iTLS) and significantly enhanced immunotherapy