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Opportunities and challenges in targeting TDLNs LN-targeted immunotherapy As TDLNs are widely recognized for playing a key role in driving the immunotherapy response, it is hypothesized that the effectiveness of ICB relies predominantly on an influx of newly primed T cells into the tumor from peripheral lymphoid organs, particularly the TDLNs, rather than on reinvigorating exhausted and fully differentiated T cells that already exist in tumors (52)