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Methods and experiments Protein expression and purification The coding regions for human PD-L1 (D1: 19134) and PD-L1 (D1D2: 19239) were synthesised and cloned into pET28a by GenScript, with codon usage optimised for expression in Escherichia coli

These adducts were only detected in ESA-treated cells and were significantly reduced in ACSL1 -knockdown cells, demonstrating an ACSL1-dependent formation of oxidative adducts of ESA-derived acyl chains in neutral lipids

The reaction mixture was vortexed thoroughly and thereafter left in the dark at room temperature for 30 min

emphasized the effects of intestinal factors (such as gut microbiota, derived metabolites, and gut barrier) on osteogenesis, bone metabolism, and maintenance via the gutbone axis