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10.1002/ptr.5987 116 WangH.PengY.ZhangT.LanQ.ZhaoH.WangW.et al (2017)

Therapeutic approaches to address copper ion metabolism abnormalities and the associated cardiovascular diseases Despite high levels of research into the understanding of the role of copper physiologically and pathophysiologically, there remain uncertainties regarding the accurate measurement of copper levels and the long-term effects of copper exposure on cardiovascular health (118)

In short, when mitochondrial membrane potential is high, TMRE accumulates in the mitochondria and produces red-orange fluorescence