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To view a copy of this licence, visit About this article Cite this article Tan, F., Liu, W., Li, T

Diabetes Care 47, S158S178

The cells were left to differentiate into MDMs for 7 days, then washed with PBS, treated with 5 mM PBS/EDTA at 37C for 20 min, harvested gently with a cell scraper, counted and replated on 96- or 6-well trays at 1104 and 1106 cells per well, respectively, as described previously (31)