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The PCR reaction is carried out in a 50 microliter volume containing 1PCR buffer, 2 mM of each dNTP, 0.7 units of High Fidelity Taq polymerse, 5 primer mix, 3 primer mix and 7.5 microliters of cDNA

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The researchers showed that senescent Leydig cell accumulation is a driver of age-related testosterone decline, and that targeted elimination of these cells with FOXO4-DRI restored endocrine function