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10.1038/nature09486 Nature 132 LautenschgerJ.SchierleG

Samples were incubated for 20 min at 37 C and subsequently diluted 1:1 with water

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Notably, Musicki and colleagues demonstrated that O-GlcNAcylation of eNOS at Ser-1177 prevents its activation by shear stress and VEGF signaling, highlighting a direct mechanistic link between PTM dysregulation and penile vascular dysfunction[54]