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The nuclei were stained at RT for 10 min without light by 4, 6-diamidino-2-phenylindole (DAPI)

Increasing the photostimulation time and further subdividing mitochondria proximal to the spot stimulation point to within 1.5 m of the spot stimulation point (denoted as spot, compared to proximal of less than 5 m and distal as over 15 m away) revealed a marked increase in OMM-HyPer7 oxidation that remained for nearly 10 min (Fig