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Ethics declarations Human and Animal Rights All reported studies/experiments with human or animal subjects performed by the authors have been previously published and complied with all applicable ethical standards (including the Helsinki declaration and its amendments, institutional/national research committee standards, and international/national/institutional guidelines)

Typical mild reactions peak within 24 to 72 hours and usually improve with simple care, whereas spreading redness larger than a few inches, fever, or breathing difficulty requires urgent evaluation

In brief, HEPES-buffered saline containing 0.5 mm EGTA (ethylene glycol-bis(-aminoethyl ether)-N,N,N,N-tetraacetic acid)and then a digestion medium with collagenase (100 digestive units/ml) were perfused via the inferior vena cava, and isolated cells were plated on collagen I-coated coverslips in DMEM supplemented with 10% FBS