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Likewise, a synthetic gene for mScarlet3 42 carrying the N-terminal Azo-tag directly after the start Met residue (for cytoplasmic expression) was subcloned via the Nde I and Afe I restriction sites

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Thymocytes exhibit disruption of the mitochondrial [ ], and a depletion of non-oxidized GSH that was near-to-simultaneous

Mutations in both proB and proA , the first two genes in L-proline biosynthesis, provided a new pathway for -glutamyl cysteine formation via the selective interception of ProB-bound -glutamyl phosphate by amino acid thiols, likely through an S-to-N acyl shift mechanism