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We here show how Alphafold-based models for interaction 39,40 , biomolecular simulations 4143 and other structure-based computational approaches 4447 , together with experimental biophysical data based on Microfluidic Diffusional Sizing 48,49 are effective to study the modulation by phosphorylation of SLiMs focusing on the OPTN LIR -LC3B complex and its disease-related mutations

For example, exposing endothelial cells to MOTS-c in vitro attenuated dysfunction caused by oxidative stress, in part by activating AMPK and suppressing inflammatory pathways (such as the MAPK/NF-B pathway) in those cells

The distance of L-lactate oxidation from the glycolytic enzymes and its proximity to these shuttles means that L-lactate formation facilitated the net restoration of cytosolic NAD+ and the achievement of net alkaline glycolysis

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