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Description

After cell lysis, the chromatin was fragmented into 100500 bp by Bioruptor Sonicator (Diagenode), and protein-DNA complexes were immunoprecipitated by 5 g HIF-1 antibody or 2 g anti-IgG antibody conjugated with Dynabeads Protein G (Invitrogen) mix on a rotator at 4 C overnight

Trarbach et al., 2010

The nine dinucleotide loci (CpG1, CpG2, CpG3, CpG4, CpG5, CpG6, CpG7, CpG8, CpG9) in the GSTA4 promoter region (Chr6:52,977,953-52,995,284, GRCh38/hg38) were selected for DNA methylation pyrosequencing (Figure 1)