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The concentration of free quercetin was measured spectrophotometrically by measuring the ultraviolet (UV) absorbance, at max 256 nm ( 2.3.3 Morphological analysis by transmission electron microscopy (TEM) To perform Transmission Electron Microscopy (TEM) analysis using the TECNAI G2 SPIRIT BIOTWIN, dilute 0.5 L of the sample with 4.0 mL of distilled water