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Evaluation of glutathione reductase activity in colon tissue of patients with irritable bowel syndrome

Farr SA, Poon GF, Dogrukol-Ak D, Drake J, Banks WA, et al

After primary antibody incubation, slices were then washed with PBS three times and incubated with secondary antibody donkey anti-mouse IgG (H + L) Alexa Fluor 488 (1:1000, Thermofisher Scientific, A-21202) in blocking solution for 90 min