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A hindpaw withdrawal at the site the heat stimulus is directed at, which is considered a reaction to the thermal stimulus

160.8 mL of perchloric acid, react for 48 hours at 10C, cool down to 0C, add 1000 mL of water, extract (the remaining tert-butyl acetate was used as solvent extraction), neutralized with Na2CO3 to pH=8 to 9, partitioned, and then extracted with 400 mL of 1% Na2CO3 aqueous solution was washed three times, and the tert-butyl acetate layer was concentrated under reduced pressure to give 42 g of Glu(OtBu)2 as an oil in 32.4% yield

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Lymph-targeted high-density lipoprotein-mimetic nanovaccine for multi-antigenic personalized cancer immunotherapy