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These transgenic mice showed significantly reduced plasma testosterone levels (18% of normal) [124] and the IGF-I deficiency was correlated with an ultrastructural analysis of mutant Leydig cells revealing a significant developmental delay, with fewer and smaller Leydig cells than normal

Briefly, The Cu(I)-catalyzed click reaction was initiated by adding Click-iT reaction buffer and 50 l of Azide-PEG3-biotin in 50 mM (Cayman Chemical, 23419) to each sample and incubated on a rotator for 20 min at room temperature

Our study revealed that NRF2 is highly expressed in healthy skin, which aligns with findings reported in public databases [42]