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The protein was eluted in G-buffer LCP (200 mM NaCl, 20 mM TrisHCl 7,5, 5% glycerol, 0.025% DDM, and 0.0025% CHS) containing 400 mM imidazole

Patients with diabetic kidney disease (DKD) exhibit pronounced gut microbiota dysbiosis, characterized by decreased abundance of commensal bacteria and increased prevalence of uremic toxin-producing bacteria (98) demonstrated that Roseburia intestinalis was significantly decreased while Bacteroides stercoris was markedly increased in the gut of biopsy-proven diabetic nephropathy patients, and this dysbiosis was closely correlated with parameters of lipid metabolism, glucose metabolism, and renal function