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Proceedings of the National Academy of Sciences of the United States of America, 86(16), 62306234

The marginalization of men in social scientific research on infertility

The real problem is how in the early stages of development a randomisation mechanism involves the appearance of a range of globulin patterns sufficiently wide to cover all determinants

Post-trypsinization, cells were hypotonically treated with 75 mM KCl for 5 min and stopped by 1 PBS quick wash, then pre-extracted with 0.1% Triton X-100 for 2 min, then washed once by 1 PBS, followed by 4% PFA fixation for 10 min