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Shimatzu 10AVP HPLC System (Shimatzu, Kyoto, Japan) with manual sample injector was used to run the HPLC-UV method on an isocratic gradient with mobile phase comprising of 10 mM 1-heptane sulfonate, 20 mM potassium phosphate monobasic [pH 3.1], 4% methanol, and 3% acetonitrile

Glycine has become a salt , with a negatively charged carboxylate group and a positively charged ammonium group

1 3 Historically, withdrawal was thought to be mild and self-limiting, misnamed a discontinuation syndrome

Cohesive energy Cohesive energy (E Coh ) is defined as the total energy required to separate a molecule or a solid into its individual atoms or molecules