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A prolonged oxidative or reductive stress will alter the homeostatic redox mechanism to cause cardiovascular complications

Lu B, Chen S, Guan X, Chen X, Du Y, Yuan J, et al

An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

Studies have shown that the expression of inflammatory markers such as Tumor Necrosis Factor alpha (TNF-a) and Interleukin-6 (IL-6) significantly diminish when there are high cellular levels of NAD+ and its precursors