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To understand whether such variability in thiol content is related to the stage of nodule development at which the mutants are blocked or to metabolic changes in ineffective nodules, we also analyzed the root tissue and nodules of 1- and 2-week-old plants (Figure 6B)

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The challenge with postbiotics is ensuring they reach relevant tissues in active form and determining optimal dosages (levels of these compounds in a healthy persons gut can be quite high locally, which is hard to replicate with oral dosing due to absorption and metabolism)