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Total RNA from worms was isolated using TRI reagent (Sigma-Aldrich)

Marc, J., Mulner-Lorillon, O

The development time should not be more than 2-3 minutes after burning starts

Akt also inactivates the pro-apoptotic Bcl-2 family member BAD (Bcl2/Bcl-XL-antagonist causing cell death) by phosphorylation, thus stopping its interaction with and blockade of the pro-survival Bcl-2 family members Bcl-2 and Bcl-XL (Papadia and Hardingham, 2007)