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Real-time quantitative PCR, triglycerides, and Oil Red O staining Intramuscular preadipocytes isolation and plasmid construction Intramuscular preadipocytes of the longissimus dorsi muscle were isolated from calves of Qinchuan cattle at National Beef Cattle Improvement Center Breeding Farm using 0.2% type II collagenase (1148090, Sigma), following the protocol described in our laboratorys previous studies [30,31,32]

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Microglia and Neuroprotection

HCV protein has been validated to hijack the patients lipid and glucose metabolism by stimulating de novo lipogenesis, promoting synthesis of phospholipids and sphingomyelins, inhibiting mitochondria fatty acid oxidation, and hijacking the very low-density lipoprotein (VLDL) secretion pathway