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Relative transcripts levels were calculated using the modified Ct method (Pfaffl, Determination of the expression of the KLIKK proteases in vivo Patients with diagnosed chronic periodontitis attending the Clinic of Periodontology at the University Hospital of Jena were recruited for this study

Because Glutathione may deplete with age, conditions related to reduced GSH levels can be considered age related

That's really what made it

The mitochondrial isoform of glutaredoxin (Grx2) is a glutathione-dependent oxidoreductase that is involved in glutathionylation/de-glutathionylation of proteins