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Additional biochemical assays were used to test the ability of compounds to inhibit nicotinamide phosphoribosyl transferase (NAMPT) and NAD+-dependent protein deacetylase sirtuin 1 (SIRT1), two enzymes in the NAD+ biosynthesis/salvage pathway

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In controlled laboratory models, researchers may evaluate how this peptide relates to fragment-specific pathway activity, peptide signaling, and experimental cellular response behavior

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