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Key assays included senescence-associated beta-galactosidase (SA--gal) activity, RT-PCR, western blotting, immunofluorescence, live-cell confocal imaging with fluorescent probes, flow cytometry and mitochondrial respiration analysis

CrossRef Profil Morbiditas Pasien Lansia Di Kota Bandar Lampung : Studi Cross Sectional Multicenter Nurul Irna Windari, Dirga Dirga, Rizky Hidayaturahmah, Putri Amelia Rooswita Jurnal Epidemiologi Kesehatan Komunitas.2025

To test whether this result is transferrable from 2D fibroblast culture to 3D human skin models, we extracted RNA from EFT-412 skin tissues following two-week MB treatment at 0.1, 0.5 and 2.5 M concentrations