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Finally, the sections were coverslipped using ProLong Gold Antifade Reagent (Thermo Fisher Scientific, Waltham, MA, USA)

The expulsed chromatin, coated with antimicrobial proteins, forms NETs (neutrophil extracellular traps), able not only to trap pathogenic microorganisms, but also to propagate inflammation and to favour the induction of microclots following platelet binding (144, 145)