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N Engl J Med 369(4):341350 Doody RS, Thomas RG, Farlow M, Iwatsubo T, Vellas B, Joffe S, Kieburtz K, Raman R, Sun X, Aisen PS, Siemers E

The combined effect of the NH 4 + and K + cations maintains the high ratio of specific to nonspecific primertemplate binding during the annealing step of every PCR cycle (see figure: "NH 4 + and K + cations in QIAGEN PCR Buffer increase specific primer annealing")