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The protein complexes were then eluted with 100 L of elution buffer containing 50 mM BisTris, pH 6.8, 500 mM NaCl, and 500 mM imidazole and subjected to SDS-PAGE analysis

Targeted treatments are urgently needed to prevent cytokine storms, and the primary infection with minimal or moderate symptoms is the critical time for aggressive therapies to control subsequent degeneration (Table 1, Figure 7) (Siddiqi and Mehra, 2020)

47 This drift correlates with reduced transcriptional coordination and increased expression variability, especially for ECM remodeling genes