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For those with inflammatory bowel disease, celiac disease, SIBO, gastric bypass history, or chronic GI symptoms that may impair oral nutrient absorption

This allowed the use of algorithms that cannot process missing values, while still using all of the dataset

293T cell culture supernatants were applied to 12% SDS-PAGE gels without prior dilution and transferred to nitrocellulose membranes (Millipore, Watford, UK) by electroblotting at 400 mA, 1.5 h, 4 C